“With MBioSEQ Ridom Typer, microbiologists can harness the full potential of next generation sequencing, transforming raw data into actionable insights, effortlessly and securely.”
Next-generation sequencing in microbial genomics
NGS has rapidly transformed the field of microbial genomics, offering greater speed and resolution for decoding bacterial genomes than traditional sequencing methods, which can be hampered by limited throughput, slower processing times and higher costs. Given the escalating threat of infectious disease outbreaks, and their profound implications for patient care and healthcare costs, there is a critical demand for rapid, accurate detection. NGS is enabling clinicians and public health teams to swiftly identify pathogens, including novel and drug-resistant strains, track outbreaks in real-time, and make informed infection control decisions. Integrating NGS into healthcare practice is becoming key to global infection prevention and control strategies.
Whole genome sequencing is a comprehensive approach that enables researchers and clinical microbiologists to perform rapid, cost-effective and robust sequencing of whole bacterial genomes. It plays a critical role in tracking transmission routes of pathogens, identifying emerging bacterial strains and uncovering antimicrobial resistance patterns, moving beyond what’s possible with traditional methods. By delivering timely, in-depth genomic insights, WGS empowers a rapid public health and clinical microbiology response to infectious disease challenges. However, the sheer volume and complexity of data require sophisticated yet accessible tools to translate raw sequences into actionable information.
Bruker’s MBioSEQ Ridom Typer: a comprehensive data analysis solution
The MALDI Biotyper is a microbial identification system based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, which identifies microorganisms to the species level with unequalled speed and accuracy. Following identification, the IR Biotyper, a Fourier transform infrared (FT-IR) spectroscopy system, can be deployed for real-time strain typing and fast outbreak detection.
To deepen analysis, integration with the MBioSEQ Ridom Typer enables identified microorganisms and outbreak suspects to be reflexed to WGS for the detection of resistance genes, specific mutations, and confirmation of strain identity. The IR Biotyper’s intuitive visualization and traffic-light reporting facilitate rapid interpretation and decision-making bolstered by the robust, comprehensive genomic data analysis from the MBioSEQ Ridom Typer. This integrated approach takes microbial monitoring to a new level, enabling quick and smart decisions.
Compliance by design
For laboratories requiring stringent data governance, the MBioSEQ Ridom Typer features configurable user roles and robust access controls that ensure the data remain local and secure within this highly maintained database. Optional collaborative efforts are enhanced through the Consortium Server, which enables secure data sharing and real-time alerts of clonal matches among users.
An intuitive 4D visualization tool presents WGS and amplicon data by person, place, time, and microbial type, facilitating clear interpretation and supporting timely epidemiological investigations. These interconnected, exportable views are publication-ready and ideal for comprehensive reporting.
Integration into microbial identification workflows
Designed to automate and simplify the entire workflow, from identification to epidemiological insight, the MBioSEQ Ridom Typer software equips laboratories with an intuitive platform that creates a seamless microbial journey with automated data analysis.
Bruker’s microbial identification ecosystem enables a seamless multi-tiered workflow, starting with rapid species identification using the MALDI Biotyper, progressing through fast strain typing with the IR Biotyper, and culminating in NGS typing and antimicrobial resistance profiling using the MBioSEQ Ridom Typer. Together, these complementary technologies create a highly efficient workflow.
The MALDI Biotyper identifies microorganisms at species level. The IR Biotyper is a rapid and cost-effective first-line screening tool for prospective outbreak detection, and MBioSEQ Ridom Typer provides unmatched genomic depth and resolution when more detailed insights are required.
Multi-drug resistant samples identified by the IR Biotyper as epidemiologically significant can be referred to MBioSEQ Ridom Typer for WGS, allowing detailed phylogenetic analysis, core genome multilocus sequence typing (cgMLST) and antimicrobial resistance – widely applied and proven effective by thousands of publications in peer-reviewed journals.
By automating complex genomic analyses and ensuring robust data security, MBioSEQ Ridom Typer enables laboratories to advance microbial typing, track infectious disease outbreaks, decode antimicrobial resistance and support hospital hygiene with unprecedented efficiency and confidence.
For more information on the MBioSEQ Ridom visit: https://www.ridom.de/ridom-typer/ and to see the applications and benefits of the IR Biotyper visit: https://www.bruker.com/en/applications/microbiology-and-diagnostics/diagnostics-solutions-and-healthcare-challenges/hospital-hygiene.html
